| dc.description.abstract | Dengue virus (family Flaviviridae, genus Flavivirus) is responsible for dengue fever and dengue hemorrhagic fever which are concerned as health burden around the world. Lanciotti protocol has been applied widely and known as a fast and accurate method in serotyping and diagnosing dengue virus by using
RT-nested PCR. However, there was an unpublished report from Ho Chi Minh
– Pasteur Institute, about misbinding of Lanciotti TS4 inner primer to DENV-1 that cause confusion in the result. Therefore, the aim of this study is to verify the issue and identify its reason. Six strains include two strains for each group: normal DENV-1, suspected DENV-1 and DENV-4 were selected. Virus isolation and reverse transcriptase-polymerase chain reaction were manipulated simultaneously in order to assure the serotype of samples. The following sequencing step finally found five mutations on mutant strains which are potentially main causes of the issue. Together with giving reasonable explanations about the binding formation between TS4 and DENV-1, this study also contributing useful information about variation in dengue 1 virus genome thus mention about the necessary of designing a new primer for the improvement of accuracy in dengue diagnosis as well as recommend an expansion of the sample size for further research.
Key words: Dengue virus, DENV-1, Lanciotti primer, misbinding, mutation. | en_US |
