| dc.description.abstract | Baizhi (Angelica dahuricae- Fisch.ex Hoffm Benth, et Hook.f) has been used as a traditional Chinese medicine. In this research, the extraction of Baizhi turberous root using ethanol has been performed, and its separated fragments by TLC using benzene/ethyl acetate (95:5 v: v) solvent system, were examined in antimicrobial assay. These fragments were analyzed to determine their percentage in the total extract and to identify their organic compound group.
The results showed that, the total extract of cultivated Angelica dahurica in the north of Vietnam had a very clear antibacterial effect and its level increased with the concentration of 0.25, 0.5, and 1(mg/μl). Four kinds of bacteria were chosen for the test, they are Streptococcus sanguinis ATCC® 10556, Escherichia coli ATCC® 35218, Listeria monocytogenes ATCC® 19111 and Staphylococcus aureus ATCC® 25213. However, the strongest effect was observed at the concentration of 0.25 (mg/μl) on L.monocyctogens and the weakest at 1(mg/μl)
on E.coli.
In addition, using TLC technique seven fragments were isolated from the extract. Two of them which were identified that contain the antibacterial agents are fragment number 5 and fragment number 7. The two fragments contain the coumarin organic compound group and their percentages of the total extract were 4.1% and 41.6% respectively.
Key word: Angelica dahurica, Antibacterial, dried glue, Thin Layer
Chromatography | en_US |
