| dc.description.abstract | Dioscorea hamitlonii Hook. f. is scientific name also called as Dioscorea persimilis
Prain & Burkill or Hoai Son in Vietnamese. In addition to being consumed as staple
food and sweet desert soup by native ethnic minorities, Dioscorea hamitonii also being
used for the medical purposes with numerous bioactivities it showed. The extractions
of Dioscorea hamiltonii were performed using different Dioscorea hamitlonii tubers
collected from several regions in Vietnam with deionized water as solvent. This study
was conducted in order to obtain and evaluate the anti-bacterial and anti-inflammatory
potential of Dioscorea hamiltonii commercial samples extracted by water with
maceration method. In this study, total polyphenol content (TPC) of the water extracts
were determined by Folin-Ciocalteu method while total flavonoid content (TFC) of
the water extracts were quantified by Aluminum chloride method. In this study,
biological properties of Dioscorea hamiltonii water extracts were evaluated for anti bacterial activity by agar well diffusion method against bacterial strains namely
Micrococcus luteus (M. luteus), Staphylococcus aureus (S. aureus), Pseudomonas
aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae). Beside, anti inflammatory effect of D. hamiltonii extracts were also evaluated by egg albumin
denaturation method and membrane stabilization assay when there was no in-vitro
study about this characteristic of this plant. Particularly, the TPC of water extract of
BMf, BMfd, BMd and KTd samples were 0.12 mg GAE/g, 0.24 mg GAE/g, 0.405 mg
GAE/g and 0.295 mg GAE/g, respectively. The result for TFC of four extract samples
were also reported from 0.074 mg NE/g to 0.275 mg NE/g. Anti-bacterial activity of
the extracts were determined at concentration of 500 mg/ml and BMf sample gave the
highest inhibition to M. luteus (11.4 ± 0.053mm). The protection of egg albumin
denaturation of BMd sample was 62.71% at concentration of 800 ug/ml and protection
of RBC membrane from hemolysis of BMd extract at dose of 1000 ug/ml was
34.508%. Therefore, the biological activities of the extracts originated from the
different sources indicated it is important to select the Dioscorea hamiltonii for
functional and pharmaceutical field | en_US |
