In vitro regeneration through callus derived from immature leaf of two sugarcane varieties : VN84 4137 and suphanbury 7

Abstract

Sugarcane (Saccharum officinarum L.) is a major crop for sugar production and plays an important role in the tropical and subtropical economy including Vietnam. In this study, two sugarcane varieties (VN84 4137 and Sufanbury 7) was studied to establish in vitro regeneration protocol by using callus derived from immature leaves as explants. Callus cultivation was initiated on MS medium containing different concentrations of 2,4D. The highest percentage of callus induction was observed in MS medium supplemented with 3 mg/l 2,4D for VN84 4137 and 2 mg/l 2,4D for Sufanbury 7. Shoot regeneration was investigated on MS medium supplemented with different combinations of plant growth regulators, naphthalene acetic acid (NAA) and benzyl aminopurine (BAP). The best response of callus was observed on medium supplemented with 2 mg/l BAP and 1 mg/l NAA for variety VN84 4137 and 1 mg/l BAP and 0.5 mg/l NAA for variety Sufanbury 7. Rooting of in vitro shoots showed that root induction was highest when NAA was at concentration of 2 mg/l for variety VN84 4137 and 3 mg/l for Sufanbury 7.

Keywords: Sugarcane (Saccharum officinarum L.) In vitro regeneration Immature leaves