| dc.description.abstract | Aspergillus flavus
and
Aspergillus parasiticus
are the common aflatoxin
producing species that usually infect foodstuff in their production line from the field
to the storage place such as peanut, corn, cereal, etc especially in tropical country
like Vietnam. Aflatoxins which are considered as derived secondary metabolites
assigned as a group of mycotoxins produced by several species of the
Aspergillus
spp
are potent hepatotoxins, immunosuppression, carcinogen that lead to mortality
or reducing the productivity of farm animals. There has been a demand for effective
method to detect these two species on dried food. In previous study, a multiplex
PCR method were designed to improve the detection process of
A.
flavus
and
A.
parasiticus
and that method showed high sensitivity and specificity by being applied
on artificially infected dried peanut. In this study, that multiplex PCR method would
be evaluated by testing the presence of
A. flavus
and
A. parasiticus
on natural dried
peanut kernels. On this purpose, the presence of
A. flavus
and
A. parasiticus
on the
collected peanut from the market was determined using two method, the
conventional culturing method in Institute of Hygiene and Public Health (IHPH) and
the mentioned multiplex PCR. The efficiency of multiplex PCR method would be
evaluated by comparing fungi detection result of two methods using appropriate
statistical tests. The result showed that 54% results from PCR method was the
same with culturing method, and after fungal enrichment by incubating peanut
kernels with distilled water overnight, this percentage increased to 76% which was
suggested not to be significantly different with culturing method. Therefore, this
multiplex PCR method was more advanced than the culturing method in detection
of
A.
flavus
and
A. parasiticus
on foodstuff.
Key words
Aspergillus flavus
Aspergillus parasiticus
Culturing method
Dried peanut kernels
IHPH
Multiplex PCR method | en_US |
