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dc.contributor.authorThao, Nguyen Thi Thu
dc.date.accessioned2015-09-22T08:11:53Z
dc.date.accessioned2018-06-04T02:25:56Z
dc.date.available2015-09-22T08:11:53Z
dc.date.available2018-06-04T02:25:56Z
dc.date.issued2014
dc.identifier.urihttp://10.8.20.7:8080/xmlui/handle/123456789/1543
dc.description.abstractRecent years, several studies related to hydrogels for biomedical applications have been much attractive due to their potential performances in drug/cell delivery and tissue regeneration. In this study, we introduce two kind of chitosan-based hydrogels for tissue adhesion, which were prepared in enzymatic mediation: Tetronic-grafted chitosan (TTeC) and Tyramine-polyethylene glycol-grafted oxidized chitosan (oxidized chitosan-PEG-TA). These chitosan-based hydrogels could be expected enhancing the interconnection between hydrogel layer and collagen in tissue via Schiff-base or electrostatic reaction. 1H NMR spectrum indicated that tyramine-tetronic and tyramine-polyethylene glycol was conjugated to chitosan and oxidized chitosan, respectively. The hydrogels underwent a rapid in situ formation within couple of seconds in presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). In vitro cytocompatible experiments with live/dead essay showed that the injectable hydrogels was highly biocompatible. Evaluation of tissue adhesion was performed on porcine skin giving the maximum tissue adhesive forces are 5 kPa for chitosan hydrogel and 35 kPa for oxidized chitosan hydrogel. This potentially obtained result could pave a way to further study and apply the oxidized chitosan-based hydrogel for wound sealant. Keywords: Chitosan Horseradish peroxidase Tetronic Tissue adhesion Tyramine Injectable hydrogelsen_US
dc.description.sponsorshipDr. Tran Ngoc Quyenen_US
dc.language.isoen_USen_US
dc.relation.ispartofseries;022001254
dc.subjectChitosanen_US
dc.titlePreparation of biocompatible and bio-adhesive chitosan-based hydrogel for biomedical applications +CDen_US
dc.typeThesisen_US


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