| dc.description.abstract | The antibacterial activities of twelve different wood decay fungi isolated in Southern Vietnam was investigated to find out the new potential antibiotics produced by macro fungi. The antibacterial activities of the collected fungi isolates were tested by three screening methods against seven different strains of bacteria: Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Vibrio cholerae, and Salmonella typhi. The screening tests were performed to identify the source of antibiotic production: as intracellular metabolites, extracellular metabolites, or metabolite accumulated on surface of mycelium. To determine the activities of antibacterial compounds from collected fungi, the fungi isolates were grown on culture substrates to promote mycelium formation. Antibacterial activities of the selected fungi was tested by disc diffusion method and confirmed by MIC determination using crude extract of the mycelium harvested from the substrate cultures. Four fungi species identified with strong antibacterial compounds produced by their mycelium including Pycnoporus sanguineus, Flavodon flavus, Fomitopsis ostreiformis and Meripilus giganteus. Crude extract of Pycnoporus sanguineus showed strongest antibacterial activity, with 7/7 bacteria strains inhibited. Fomitopsis ostreiformis antibacterial activity was recognized with the ability to inhibit 7/7 bacteria strains but with higher concentration. Flavodon flavus and Meripilus giganteus showed weaker activity than other two species, with only 4/7 strains inhibited. Chromatography of Pycnoporus sanguineus crude extract revealed the antibacterial activities of this fungus comes from a group of flavonoids. The fungi isolates identified with antibacterial activity discovered in this study can be the potential candidates for the new source of antibiotic to fight against new generations of antibiotic resistance bacteria in the future. Keywords: Antibacterial activity, wood decay fungi, Pycnoporus sanguineus, Flavodon flavus, Fomitopsis ostreiformis, Meripilus giganteus, Sounthern Vietnam. | en_US |
