| dc.description.abstract | In general, exopolysaccharides (EPS) from lactic acid bateria (LAB) have many applications in food industry, and pharmaceutical field. Nowadays, Lactobacillus rhamnosus and Lactococcus lactis are mainly used in pharmaceutical field. In order
to find the role to improve the EPS production in these LAB, two strains of L.rhamnosus and L. lactis were co-cultured together. After selection, each strain cultured individually, and then the concentration of EPS was measured. To purify EPS, the ethanol-precipitation method was performed. The ethanol-precipitation method was used to isolate EPS from medium. After that, this experiment used phenol-sulfuric acid assay and spectrophotometer in order to estimate the concentration of EPS. The SDS-PAGE was used to separate proteins in these strains before and after co-cultured to compare the differentiation between them. The concentration of EPS in two strains after co-cultured was higher than before co- cultured. Besides that, there were the changes of morphology and proteins of each strain after co-cultured. So, the result suggested that there were the differences of proteins, the changes of EPS’ concentration in L. rhamnosus and L. lactis before and after co-cultured following the altered protein expression that might affect on EPS
production. | en_US |
