| dc.description.abstract | Diabetic patients display increased risk of diabetic foot ulcer and chronic wound, mainly
caused by the persistence of hyperglycemia. Chronic wounds are one of the most common
complications. Platelet products provide various growth factors and cytokines for chronic
wound healing. In previous research, adipose tissue-derived mesenchymal stem cells (ATMSCs) have demonstrated as an in-vitro wound healing model by their ability to cell
proliferation, migration, angiogenic. In this study, we investigated the effect of activated
PRP (platelet-rich plasma) and their by-product activated PPP (platelet-poor plasma) on
the wound healing in-vitro of AT-MSCs under high D-glucose conditions. PRP and PPP
were activated by Thrombin and Calcium chloride. The cell proliferation was determined
by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay for 12
days. In-vitro scratch assay was used to examine the healing ability of AT-MSCs under the
influence of high D-glucose concentrations. The results shown that the cell proliferation of
AT-MSCs cultured with 10% PRP and 10% PPP were significantly higher than those with
10% FBS after 12 days. Moreover, the migration ability in-vitro was induced significantly
in 10% PRP-treated AT-MSCs under high D-glucose conditions. Our research
demonstrates the potential of PRP on the inducing wound-healing capacity of AT-MSCs
under the impact of high D-glucose levels. This work gives a better knowledge of the
influence of PRP and PPP on the activities of AT-MSCs under high D-glucose conditions
for future applications of AT-MSCs in the treatment of diabetes complications. | en_US |
