Combination Of Visible And Infrared Light-Emitting Diode (Led) Therapy To Treat Gout In Mouse Model

Abstract

Gout is a significant health concern because it causes a high level of illness, specifically intense pain, and can also lead to death due to its connection with metabolic syndrome, coronary heart disease, and diabetes mellitus. It is important to identify and diagnose the disease early in order to begin timely and suitable treatment, and to minimize complications such as joint damage, tendon rupture, and kidney and heart problems that can occur if the diagnosis is delayed. The traditional method of diagnosing gout involves examining the patient's symptoms, conducting lab tests, and analyzing joint fluids, while imaging is used as a supplements. Therefore, the objective of this study is to evaluate the effects of three wavelengths specifically 940 nm, 650 nm, and 780 nm on treatment the right leg of rats with hyperuricemia. A pulse wave generator was designed and built with three different light sources including wavelength of 650nm (60%), 780nm (40%), and 940nm (60%), and then experimented on mice model. Experiments were performed on five groups of mice with different treatments including (1) completely with diode laser (8 mice), (2) completely with drugs (9 mice), (3) completely with Perilla leaf herbal medicine (7 mice), (4) combining drugs with diode laser (7 mice), (5) combining oral administration of Perilla leaf herbal medicine with diode laser (7 mice), (6) completely do not apply any therapy at all (6 mice), by University Research Institute of Medicine and Pharmacy designed and processed in 15 days. Therapeutic dose was once daily for 7 days for all groups. After each day of treatment, the circumference of the ankle joint of the mice will be measured compared to before the disease and recorded the data combined with blood collection on the last day of treatment to be able to give the most accurate results. The results showed that when combining drugs with diode laser irradiation gave the best treatment effect. The highest swelling reduction rate reached 18 %, the highest among 6 treatments, completely with diode laser treatment method on mice also gave very positive results at 8 %. For the group that did not apply any therapy, it was found that not applying any gout therapy did not reduce uric acid levels and the mouse leg size also increased by 2 %, making the disease worse. This study demonstrates that the application of low-level laser-infrared light therapy (LLLT) to the treatment of gout in a mouse model is very promising in non-invasive phototherapy.