| dc.description.abstract | Tissue engineering, which combines biomaterials, bioactive chemicals, and cells, is a
promising technology being studied globally to become an effective and sustainable
method to treat the problem of organ failure and organ transplant shortage. In this
work, we developed an in-situ hydrogel system that modified and combined two
components at varying mixing ratios, N-Succinyl chitosan (NSC) and Oxidized
alginate (OA), through Schiff base crosslinking. The hydrogel system contains
biphasic calcium phosphate (BCP) and ascorbic acid (AA), which could enhance
biological characteristics and accelerate bone repair. The hydrogels’ properties were
examined through physicochemical tests such as Scanning electron microscopy
(SEM), Energy-dispersive X-ray spectroscopy (EDS), Fourier-transform infrared
spectroscopy (FTIR), X-ray diffraction (XRD), pore size and porosity measurement,
swelling ratio, degradation rate, ascorbic acid release study, as well as biological
characterization, includes live/dead and cytotoxicity assays. The results reveal that the
effects of AA and BCP components can reduce the physico-mechanical properties of
the hydrogel system such as compressive strength, pore size, porosity, swelling ratio,
etc. However, they show biocompatibility and non-cytotoxic properties. Overall, the
results demonstrate that the hydrogel composed of 3% (w/v) NSC, 3% (w/v) OA
(NSC: OA volume ratio is 8:2) loaded with 40% (w/w) BCP and 0.3 mg/ml AA has
the potential for bone regeneration. | en_US |
