Study Of Antimicrobial Activities Of Berberine Nanoparticle In Combination With Piper Betel Extract

Abstract

Bacteria bear a significant burden in healthcare environments since they cause various health problems, including infections, particularly those that cause gastroenteritis (Salmonella, Shigella, E. coli, Methicillin-Resistant S. aureus, etc.), develop as biofilms, and are frequently accompanied by high rates of mortality. The use of antibiotics treatments to treat and prevent microorganisms is associated with the capability to cause antibiotic drug resistance. Consequently, there has been a huge demand for and vital relevance of the rapid and efficient method of preventing the impacts of bacteria without medication. In this work, a novel approach to combining herbal ingredients and natural materials with nanostructured lipid carriers loaded with berberine has been designed. Piper betel is high in phytochemicals and essential oils, which have been demonstrated to have potently increasing antimicrobial properties. Berberine is prepared in nano-formulation to improve its solubility and increase its antibacterial effectiveness against hospital-acquired infections. The investigations were carried out to determine the antibacterial activity of Piper betel leaf extract in three different solvent types (distilled water, 70% ethanol, and 95% ethanol) produced by the high-pressure homogenization method, berberine-loaded nanostructured lipid carriers, which were produced by high-pressure homogenization, and the antimicrobial efficacy of Piper betel leaf extract combined with berNLC. Even though both approaches effectively contribute to antibacterial activity, the results showed that combining ber-NLC by the high-pressure homogenization method with betel leaf extract as a reducing agent was more promising in terms of antibacterial activity. The development of antibacterial products based on ber-NLC combined with Piper betel extracts will be especially dependent on this result.