Investigation of vitamins production in lactic acid bacteria

Abstract

Lactic acid bacteria (LAB) are safety microorganism, used as probiotic for human. They are capable of synthesizing essential biomolecules such as vitamins, enzymes, exopolysaccharides, bacteriocins or bioactive peptides serve into the functional and technological properties of the products. The purpose of this study is investigation the production of vitamins by lactic acid bacteria originated from plant and look for the conditions for vitamin production. To our knowledge, this is the first study to detect B ±group vitamins produced by Lactobacillus. By high perform liquid chromatography analysis, the pyridoxine produced in Lactobacillus rhamnosus PN 04 cultured in MRS broth equaled to 0.062 mg/ml and folic acid amounts produced in Lactobacillus rhamnosus PN04 cultured in fish medium gave 0.002 mg/ml, respectively. Lactobacillus fermentum could produce riboflavin in fish medium (0.005 mg/ml). Otherwise, Lactobacillus fermentum did not produce any vitamins in MRS and fish gills-extracted media. The results were also confirmed using thin-layer chromatography analysis. Pyridoxine produced in Lactobacillus rhamnosus was detected. As a result, Lactobacillus rhamnosus PN04 and Lactobacillus plantarum could be the vitamin sources for pyridoxine, folic acid and riboflavin production in this reseach. Key words: Lactic acid bacteria (LAB) Vitamin Optimum