Isolation and identification of yeast and bacteria that produce cellulase and pectinase activities from coffee plant

Abstract

Cellulase and pectinase are used in various fermentations as coffee, pepper, or cocoa for mucilage removal and also flavor enhancement. However, the sources of microorganisms are important, so in the study, the purpose is to isolate yeasts and bacteria producing these enzymes. In this study, the purpose is to isolate yeast and bacteria that can produce cellulase and pectinase. Therefore, the research mainly found yeasts and bacteria in the coffee plant can produce cellulase and pectinase activities. Seven strains of yeasts and bacteria were determined including Candida quercitrusa, Klebsiellaoxytoca, Erwiniasp, Enterobacter cloacae, Gluconobacteroxydans, Enterobacterludwigii, Erwiniabillingiaewhich isolated in coffee plant. This is the first step showed microorganism sources that isolated from components of coffee plant had enzyme activities. From that, it can be concluded that this study may be applied well in the conditions of the production of the enzyme were studied and make enzyme production. Key Words: Cellulase, Pectinase, coffee plant andsubstrate.