| dc.description.abstract | Auxin group and cytokinin group were examined for the effects of
micropropagation of Oncidium orchid by PLBs techniques, with purposes of
preservation of Vietnam breeds and supplement for demand of customers. MS was
used for all experiments. It contained essentials elements, 10% (v/v) coconut
water and 20 g/L of sucrose. Callus induction from shoot clusters of Onc. orchid
obtained from 2,4D with concentration of 1 mg/L for the highest frequency
induction (76.19%) and 0.25 mg/L for the biggest size of callus (8.02 mm). At the
concentration of 0.75 mg/L NAA, the highest number of PLBs was achieved (98
PLBs) from callus clusters of Onc. orchid after 90 days of culture. Besides, 28.18
PLBs were the best result obtained from combination of 0.50 mg/L NAA and 0.50
mg/L BA from shoot cluster of Onc. orchid. Moreover, PLBs micropropagation could
be gotten maximum number of PLBs at 1 mg/L NAA (79.21 PLBs) from start
protocorm - like - bodies explants. Shoot induction and regeneration were
afterward initiated. The best result was recorded at 1 mg/L BA (12.42 shoots) and
combination of 0.25 mg/L BA and 0.25 mg/L NAA (11.66 shoots) from shoot
explants. Shoots were 3 - 5 cm height and had 6 - 9 leaves. Finally, root
generation was performed. NAA at 0.75 mg/L gave the highest number of root
formation (2.64 roots) and at 1 mg/L NAA, it gave the longest length of root (12.58
mm).
Key words: 2,4D, BA, callus, micropropagation, NAA, PLBs, root, shoot | en_US |
