Preparation of biocompatible and bio-adhesive chitosan-based hydrogel for biomedical applications

Abstract

Recent years, several studies related to hydrogels for biomedical applications have been much attractive due to their potential performances in drug/cell delivery and tissue regeneration. In this study, we introduce two kind of chitosan-based hydrogels for tissue adhesion, which were prepared in enzymatic mediation: Tetronic-grafted chitosan (TTeC) and Tyramine-polyethylene glycol-grafted oxidized chitosan (oxidized chitosan-PEG-TA). These chitosan-based hydrogels could be expected enhancing the interconnection between hydrogel layer and collagen in tissue via Schiff-base or electrostatic reaction. H NMR spectrum indicated that 1 tyramine-tetronic and tyramine-polyethylene glycol was conjugated to chitosan and oxidized chitosan, respectively. The hydrogels underwent a rapid in situ formation within couple of seconds in presence of horseradish peroxidase (HRP) and hydrogen peroxide (H O ). In vitro cytocompatible experiments with live/dead essay showed 2 2 that the injectable hydrogels was highly biocompatible. Evaluation of tissue adhesion was performed on porcine skin giving the maximum tissue adhesive forces are 5 kPa for chitosan hydrogel and 35 kPa for oxidized chitosan hydrogel. This potentially obtained result could pave a way to further study and apply the oxidized chitosan-based hydrogel for wound sealant. Keywords: Chitosan Horseradish peroxidase Tetronic Tissue adhesion Tyramine Injectable hydrogels