Study on the injectable cationic hydrogel for heparin delivery
Abstract
In recent years, injectable cationic hydrogels have been widely studied towards
biomedical applications because of their potential performance in drug/cell delivery. In
this study, we introduce a simple and organic solvent-free method to prepare
tyramine-tetronic via activation of four terminal hydroxyl groups of tetronic, tyramine
conjugate
into
the
activated
product
to
obtain
Tetronic
±TA
(TTe
)
and Denrimer PAMAM G3.0 was functionalized with Hydroxyphenyl acetic acid (HPA)
by use
of carbodiimide
1
-
ethyl-3-(3-(dimethylamino)propyl)carbodiimide
(EDC)
coupling agent to obtain PAMAM - HPA. The polymers were well characterized by -
1
HNMR. The aqueous TTe and PAMAM-HPA copolymer solution rapidly formed
hydrogel
in
the
presence
of
horseradishperoxidase
enzyme
(HRP)
and
hydrogenperoxide (H O ) at physiological conditions. The gelation time of the
2
2
hydrogel could be modulated ranging from 7 to 73 sec, when the concentrations of
HRP, H O , and polymers varied. In vitro cytotoxicity study with Human Foreskin
2
2
Fibro-blast cell
using
live/dead
assay indicated
that
the
hydrogel
had
high
cytocompatibility and could be used as a drug delivery material. Heparin release
profiles show that the dendrimer- base hydrogel or cationic hydrogel could control
release of heparin as compared to control sample. The obtained results demonstrated
a great potential of the cationic hydrogel in biomedical applications.
Keywords:
Cationic hydrogel
Tetronic
Dendrimer G3.0
Injectable