Effects ionomycin and 6-demethylaminopurine on the parthenogenetic development of in vitro matured porcine oocyte

Abstract

The aim of this study was to survey the optimal time for treatment of ionomycin and 6-dimethylamnopurine (6-DMAP) in parthenogenetic embryo activation. For ionomycin testing, matured oocyte was treated with ionomycin in 3 different duration (1, 5, 20 mins) and 6-DMAP in 4 hours , A control sample was run alongside treatments without using ionomycin. Results in cleavage ratio was obtained in treatments at 1, 5 20mins vs control (23.879% , 70.597 , 83.824 vs 4.17 8) . Ratio of block ( 20.505% ,78.978% , 37.210% vs 0%) and ratio of blastocyst ( 0% , 15.051% , 4.231% vs 0%) . For 6-DMAP testing, the best result from ionomycin treatment was applied as standard time ( 5mins). After matured oocyte was treated with ionomicin (5mins) , they were treated with 3 different duration ( 1, 4,10 hs), A control sample was run alongside treatments without using 6-DMAP. The results in cleavage ratio obtained in the treatments at 1, 4, and 10hs vs control (22.092%, 70.597%, 13.764% vs 11.970%) , block ratio (62.179%, 78.978%, 0% vs 0%), blastocyst ratio ( 3.905%, 15,051%, 0% and 0%). It was concluded that association of ionomycin at 5 minutes and 6- DMAP at 4 hours was the best time duration for blastocyst development Keyword: porcine, oocyte activation, cleavage ratio, block ratio, blastocyst ratio, parthenogenesis