Evaluation of antimicrobial avtivity of glinus oppositifolius L

Abstract

The aim of this study was to isolated and identified the phytoconstituents which are responsible for antimicrobial activity in Glinus oppositifolius L. The chemical constituents were isolated and identified by means of chromatographic and spectroscopic techniques methods. The phytochemicals analysis of tested plant which was based on standard protocol showed the presence of tannins, glycosides, amino acids, steroids, saponins, alkaloid, and flavonoids. Ethanol and methanol extracts were tested for their antimicrobial activity on 4 different species of bacterial : Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The methanol and ethanol extracts exhibited significant antimicrobial, comparable to the standard drug Norfloxacin. Minimum inhibitory concentration (MIC) test which was done by broth dilution method resulted in the ability to inhibit the survival of 4 kinds bacterials at 2100 µg/ml (ethanol extract), and 1800 µg/ml (methanol extract). Total phenolic (675.1 ± 8.03 mg of GAE/g) and flanovoid (111.3 ± 5.48 mg/g of sample) contents were also determined. β-sitosterol-3-O-β-D-glucopyranoside was purified and identified by column chromatography. Keywords: Glinus oppositifolius L, β-sitosterol-3-O-β-D-glucopyranoside, total phenolic, total flavonoid, antimicrobial, MIC.