Expression of hemagglutinin, neuraminidase and M1 matrix proteins CHO-K1 cell line

Abstract

Hemagglutinin (HA), neuraminidase (NA) and matrix protein (M1) are three main influenza structural proteins which play roles as antigen when they enter host immune system. Because of their characteristics, HA, NA and M1 have a promised application in vaccine development and production. The purpose of this study has been reached when we obtain a modified cell-line which has ability to express the three proteins stably. The surface glycoproteins HA and NA and the matrix protein M1 were produced in China hamster ovary (CHO) cells by co-expressing three genes NA, HA and M1 using pZIC-HA, pZIC-NA and pZIC-M1 plasmids as expression system. Three plasmids have secret sequences which enable cells to secret HA, NA and M1 outside into culture medium. Subsequently, cell culture medium was collected during all time of selection for preparation of test series such as HA test and HI test. The positive results from hemagglutionation (HA) and hemagglutionation inhibition (HI) test proved the modified CHO-K1 cell line which had ability to express target genes. This study identified the most suitable HA:NA:M1 ratio for gene transfection which was 1:1:1. There were first steps for advanced researches about HA, NA and M1 gene transfection in the future. Keywords: CHO-K1 Hemagglutinin Lipofectamine Matrix protein M1 Neuraminidase Type A influenza virus