Preparation and biological activity of calcium carbonate nanoparticles

Abstract

Combined nano calcium carbonates( CaCO3) and Cholecalciferol (Vitamin D3) have been successful synthesized by using the bottom-up method with sizes ranging from 50-500nm. Nano-calcium carbonates were analyzed by field- emission scanning electron microscopy (FE-SEM) and powder X-ray diffraction (XRD). The effects of different concentrations between CaCl2.2H2O and Na2CO3; the influence of the amount of PEG6000 on the sizes and shapes of precipitated particles of CaCO3 were investigated. As result, acute toxicity with dose from 0.33, 0.65 to 1.30g/kg body weight and sub-chronic oral toxicity test with dose of 1.30g/kg body weight were tested to establish the safety of this product for long-term oral administration. In vivo results of serum calcium concentration of mice studies indicated that orally administered nano-calcium carbonate/vitamin D3 can enhance the serum calcium concentration. It could be concluded that in nano calcium carbonate is more bioavailable than in micro calcium carbonate in test mice. Keywords: Nanoparticle; CaCO3; vitamin D3; PEG 6000; serum calcium.