Evaluation the micropropagation of ehretia asperula zoll. Et mor

Abstract

Studying about the micropropagation of Ehretia asperula Zoll. et Mor, Seven experiments were carried out: explant sterilization, node sprouting, shoot elongation, shoot formation, shoot regeneration from leaves callus, effect of pH on node sprouting, and root formation. Results of experiments showed that surface sterilization using alcohol 70% in 1 minute, Javen 20% in 15 minutes, HgCl2 0.1% in 5 minutes, three times with sterilized distilled water was the suitable condition (our research obtained 87.5% single node sprouting). MS medium supplemented with Kinetin 0.5 mg/L and BA 0.5 mg/L was the suitable for sprouting medium. Then, on the MS medium supplemented with BA 0.1 mg/L, the shoot length could be increased of 104.51% after three weeks. When shoots were cultured on MS medium supplemented with BA 0.2 mg/L, the results indicated that 22.15% explants could regenerated new shoots. As the results of experiment, shoots could not be regenerated from leaves were cultured on the MS medium supplemented with BA 0.1 mg/L + NAA (0.3; 0.4; 0.5 mg/L). In addition, our research also showed that pH level at 6.2 was suitable to increase node sprouting and stage of rooting was better to be cultured on MS 1/2 medium supplemented 0.1 mg/l of IBA. Keywords: BA, Ehretia asperula Zoll. et Mor, Micropropagation, Kinetin, MS medium, and NAA.