Production of pectinase and cellulase of penicillium citrinum cultured in molasses

Abstract

In this study, strain of fungi Penicillium citrinum was used as a major object for pectinase and cellulase production in molasses. At the room temperature (27-30°C) and initial cell concentration was 3.5x106 spores/ml, the highest pectinase activity was achieved when Penicillium citrinum was grown during 48 hours with the sugar concentration of molasses was Brix 9° used as substrate, 3% pectin and other minerals such as: 1% ammonium sulfate as nitrogen source, 0.15% urea, 0.06% phosphoric acid, 0.05% magnesium sulfate. On the other hand, at the same temperature (27-30°C) and the same mineral contents, the highest cellulase activity was achieved when Penicillium citrinum was grown with the sugar concentration of molasses was Brix 9° and 1% cellulose during 72 hours. Keywords: Cellulase Liquid submerged fermentation Molasses Pectinase Penicillium citrinum