dc.description.abstract | Lactobacillus plantarum is a non-pathogenic gram positive and rod shape
bacterium that known as a member of Lactobacillus species. Lactobacillus
plantarum has reported as a probiotic that had many applications in food
industry, but their potential were not fully investigated include the potential as
uronic acid source. In this study, the detection of uronic acid and the
antimicrobial activity of Lactobacillus plantarum were done. Amazingly, the
uronic acid was detected and measured about 0.97mg/ml by using carbazole
assay. The antimicrobial activities of Lactobacillus plantarum was shown on
Candida albicans (12mm), Salmonella typhi (6.67mm), Staphylococcus aureus
(7mm), Staphylococcus sciuri (9mm), Micrococcus luteus (15.67mm), Vibrio
harveyi (6mm), Vibrio paraheamolyticus (8.67mm) and Pseudomonas
aeruginosa (7.33mm). The temperature also showed the effect on antimicrobial
activity of Lactobacillus plantarum. At 37oC the antimicrobial activity was
strongest. The CFS from Lactobacillus plantarum was only active at pH 2 to 4
while inactive with pH above 4. The antimicrobial agent was lysed after treated
with Trypsin showed that the antimicrobial agent was proteinaceous bacteriocin.
This study reported the biological activity of Lactobacillus plantarum as a
potential application in pharmaceutical and other fields. | en_US |