| dc.description.abstract | The objective of this study is to investigate the effect of sample-water
ratio (m/v), extraction time (min) and extraction temperature (oC) on total
phenolic content (TPC) of Centella asiatica. The optimized mode (1: 100 (m/v),
90 min, 25oC) of the extraction condition was established to obtain the maximum
total phenolic content (23.2 ± 0.5 mg FAE/g DW). Moreover, total flavonoid
content (TFC) (11.2 ± 0.1 mg RE/g DW) and tannin content (13.8 ± 1.0 mg/g
DW) were determined. The ability to scavenging DPPH free radicals was also
investigated with IC50 (224.8 ± 9.2 μg FAE /ml concentration of TPC). Moreover,
TPC, TFC and tannin were considered as contributors to inhibit alpha-amylase
and alpha-glucosidase activities at a certain concentration of TPC in the extracts.
At 417.9 μg FAE/mL of TPC in extract, the amylase inhibition made up 23.0 ±
1.2% while 65.2 ± 2.4% was the percentage of alpha-glucosidase inhibition.
Spray drying using maltodextrin and gum arabic as carriers resulted in
significant loss of TPC, TFC and tannins of Centella asiatica’s extracts.
Keywords: Centella asiatica, total phenolic content (TPC), total flavonoid
content (TFC), tannin, antioxidants, alpha-amylase inhibiton, alpha-glucosidase
inhibition, spray drying. | en_US |
