Study on technological conditions for extraction of bromelian from by - products (shoot and tap root) of pineapple at Minh Dat's company

Abstract

Bromelain is a protein digested enzyme, aqueous extract that is extracted from the shoot and tap root of the pineapple. Recently, a wide range of therapeutic benefits have been attributed to bromelain such as the reversible inhibition of platelet aggregration, impoved recovery after surgical traumas, etc. The purpose of this study was to survey the technological conditions that optimizing the extraction of enzyme bromelain from waste of pineapple. It is usually prepared using centrifugation, ultrafiltration methods, and precipitation by ammonium sulfate, acetone or ethanol, and freeze-drying. At the different conditions, the bromelain is surveyed the activity of each kind of enzyme at that condition.The efficiency of the extraction of bromelain was confirm by the new Anson method and Lowry method, showing that this method could be used to check the activity of bromelain exactly. The results showed that bromelain was recovered by ammonium sulfate at 80% that get the highest activity. The bromelain extraction method using tap root of pineapple in this study yielded specific enzyme activity of 72,68UI/mg. However, in the same condition, using the shoot that getting activity better than tap root, it’s achieved 98,37UI/mg.If the study is successful,so the application of them for the food industry. Keywords: Bromelain Enzyme Ethanol Ammonium sulfate Acetone