| dc.description.abstract | Resistance to anticancer drugs that reduce efficiency of chemotherapy is a
widespread problem in cancer treatment. The subsequent development of drug
resistance can result in rapid disease progression during or shortly after completion
of treatment. There is much reasons lead to the drug resistance in breast cancer.
One of the main reasons is the heterogeneous of breast cancer cell populations.
Each subpopulation in breast cancer tumor may have separate drug resistance. This
research aims to evaluate drug resistance of these subpopulations.
In order to assess drug resistance, primary cells were cultured from breast cancer
tumor by tissue culture method. Successfully primary cultured cells were stained
with CD44 and CD24 surface markers and then sorted into 4 subpopulations: The
Origin, CD44+, CD44-/CD24- and CD44+/CD24-. Subpopulations were treated in
various concentration of Doxorubicin (0.05 – 1 µg/mL), an essential anticancer
drug, during 96 hours. The cytotoxic effect of Doxorubicin was real-time constantly
measured by xCELLigence system. Obtained results showed that drug resistance of
subpopulations is dissimilar, and the combination of resistant populations to
anticancer drug leads to the drug resistance of breast cancer tumors.
Key word: breast cancer tumors, breast cancer stem cells, CD44, CD24, drug
resistance, and subpopulation. | en_US |
