| dc.description.abstract | Bacterial biomass, particularly in vivo, is considered as an alternative source of
protein and other essential nutrients for aquaculture species given the
increasing cost and unsustainable supply of fish meal worldwide. In this study
six bacterial strains isolated from shrimp ponds (Bacillus subtilis, B.
amyloliquefaciens, B. cereus, B. pumilus, Pseudomonas pseudoalcaligenes and
P. stutzeri) were evaluated for protein and lipid contents, and protein
digestibility. The results showed that, after 48 hours of batch fermentation in LB
medium at 30°C and 150 rpm, the density of the bacterial strains were higher
than 109 cfu.mL-1. Among them B. amyloliquefaciens had the highest density of
2×1010 cfu.mL-1. The dry cell weight of all the strains was higher than 16 g.L-1.
Bacillus spp. had significantly higher protein contents compared with
Pseudomonas spp. (P<0.05). Among all the evaluated strains B. pumilus had
the highest protein content of 30.0% dry weight, P<0.05). However, the highest
lipid content was recorded in P. pseudoalcaligene (12.9%, P<0.05). Protein
disgestibility, as reflected by the degree of hydrolysis determined by pH-stat
method, were 28.5% and 26.9% for B. cereus and B. pumilus, respectively;
significantly higher than that of the others.
Keywords:
Bacillus spp.;
biomass;
lipid;
protein;
protein digestibility;
Pseudomonas spp.; | en_US |
