Antibacterial activity of combines portulaca oleracea, houttuynia cordata and euphorbia hirta linn +CD

Abstract

This study investigates antimicrobial activity and Minimum Inhibitory Concentration of Portulaca oleracea, Houttuynia cordata, Euphorbia hirta Linn and the combination of P.oleracea with H.cordata, combination of P.oleracea with E.hirta Linn, and H.cordata with E.hirta Linn. Three plants have been expressed by Ethanolic extraction. Antimicrobial activity of all combinations and each individually plant extracts was tested by using Agar well Diffusion Method and compared with ability in antibacterial of their combination from each other and standard antibiotic, ampicillin. The Minimum Inhibitory Concentration of the combined was estimated by Broth Dilution Method. Seven standard pathogen bacteria strains were tested. The extracts of P. oleracea, H. cordata, E. hirta and their combinations inhibited growth of all sevens common bacteria strains in this study. When comparing three mixtures with each individual extract, E.hirta combined with H.cordata was presented as the most potent extract in antibacterial activity. All combinations showed low MIC that is important in health care delivery system so that they can be used as an alternative orthodox antibiotic in infectious diseases. Key words: P.oleracea H.cordata E.hirta Linn Combination Antibacterial MIC