| dc.description.abstract | Neuroblastoma originates from neural crest precursors in the adrenal medulla, the
sympathetic ganglia in the neck, mediastinum, retroperitoneum, or pelvis. It is the most
common extracranial pediatric solid tumor and the most common neoplasm among infants.
The expression and degree of amplification of the MYCN oncogene in neuroblastoma is
significantly correlated with poor prognosis. In this research, we investigate fluorescence in
situ hybridization (FISH) on detection of MYCN (2p24) amplification in formalin-fixed,
paraffin-embedded and single-cell suspension sample of 10 neuroblastoma pediatric tumors
(stage IV) in which, FISH technique identified MYCN amplification in 1 case of total number
patients. The results indicate that FISH is a rapid and reliable method for detection of MYCN
oncogene amplification in routinely processed samples, thus facilitating therapeutic
decisions based on the presence or absence of this prognostically important biologic marker.
Key words: neuroblastoma, MYCN amplification, fluorescence in situ hybridization. | en_US |
