Analysis of milk protein, fat, total solid and detection of aflatoxin in milk

Abstract

Milk is a good source of nutrients such as carbohydrate, protein and fat so that milk is one of the best choices of consumption of all age groups. Contamination of milk and dairy products to aflatoxin M1 is a risk for human and it can be a public health concern. The aim of this study was to determine milk protein, fat and total solid contents by Kjeldahl, Rose-Gottlieb, gravimetric methods, respectively and evaluate the presence of aflatoxin M1 in raw milk samples from markets and farms by ELISA (Enzyme- Linked Immunosorbent Assay) technique. The results showed the average protein content of 2.7± 0.45%, 3.18± 0.64%; total solid content of 12.45± 2.13%, 13.02±2.1% from markets and farms samples respectively; fat contents from farms samples 3.9± 1.44%. In 30 markets milk samples, 23 samples was AFM1 positive without exceeding the permissible limit. In 140 farms milk samples, there was 102 AFM1 positive samples in which 9 samples was exceeding safety concentrations. Key words: protein, fat, total solids, aflatoxin, ELISA