Determination of protein markers of in vitro induced antibiotic resistant staphylococcus aureus by 2D-page

Abstract

Staphylococcus aureus (S. aureus) is a considerably pathogenic hazard in human because it was the fifth rank nosocomial infectious cause. It is also one of the most commonly isolated organisms in infectious diseases in Vietnam. S. aureus isolates from intensive care units across the country and from blood culture isolates worldwide are increasingly resistant to a greater number of antimicrobial agents. The emergence of antibiotic resistance is driven by antibiotic use, along with other factors such as the spread and fate of bacteria, resistance genes and antibiotics, as well as the behavioral and medical activities of people. The contribution of these factors produces the problem and the difficulty in reserving it. In this study, 2D-PAGE was set up and optimized to figure out potential protein markers in antibiotic resistance and would support further analysis of their roles in antibiotic resistance mechanism in future studies. This study was to be done via running 2D-PAGE and then comparing protein profiles between flouroquinolone (FQ) sensitive S. aureus ATCC 29213 to the in-vitro induced FQ resistant S. aureus ATCC 29213. Although 2D-PAGE is quite costly, this technique is powerful thus widely used in many laboratories all over the world. With this work, 2D-PAGE technique has already been established, optimized and ready to serve further research at our institution. Key word: S. aureus, 2D-PAGE, flouroquinolone, resistant