Enzyme-mediated in situ preparation of hydrogel composites from chitosan/gelatin and studies on their biodegradation for bone regeneration

Abstract

Injectable and degradable hydrogel composites are promising substrates for the bone regeneration due to its potential performances in recently reports. In this study, two derivatives of chitosan and gelatin, chitosan- 4 hydroxyphenylacectic acid (CHPA) and gelatin-tyramine (GTA) were utilized to prepare chitosan/gelatinbased hydrogels via horseradish peroxidase mediated reaction in the presence of hydrogen peroxide (H2O2). The hydrogel was used to load 10 wt% biphasic calcium phosphate nanoparticles (BCP) to producing a biocomposites. The structure of these polymers were characterized by 1H NMR, UV-vis. The in situ gel formation was varied within a time period by controlling the concentration of H2O2. Moreover, biocompatible evaluation of the chitosan/gelatin-based hydrogels were conducted on Human Foreskin Fibroblast cell using live/dead assay. The in vitro biodegradation behaviors of the chitosan/gelatin-based hydrogels and hydrogel composites were investigated for up to 4 weeks using enzyme collagenase and the weight-losses method. The obtained results demonstrated a great potential of hydrogel composite as a promising biomaterial for bone regeneration due to its adjustable gelation time, high biocompatibility and degradable property. Keywords: Bone regeneration Chitosan Gelatin Horseradish peroxidase enzyme (HRP) Biodegradation Enzyme collagenase