| dc.description.abstract | There is a relationship between Oct4 gene as a transcriptional regulatory factors and
murine preimplantation development. It shows that Oct4 expression correlates with
the pluripotent cells of the early mouse embryo. Furthermore, based on morphology
and distribution of murine endogenous Oct4 protein, people can sufficiently select
undifferentiated cell lines. However, the period of the first differentiation event in
mouse preimplantation embryo has been unclear, whether it occurs at the early,
late stage 8-cell embryos or morula stages or something else. In this study, in vivo
fertilization between ICR male and female strain was carried out, and their embryos
were cultured and stained by immunofluorescence technique, with regard to Oct4
expression. The results support previous researches that Oct4 protein expresses in
all stages of preimplantation development. Moreover, at expanded blastocyst stage,
Oct4 was down-regulated. No more Oct4 expression in trophectodermal cell, but still
maintains in inner cell mass of these expanded blastocysts.
Key words: In vivo fertilization, mouse embryo, in vitro culture, preimplantation
development, Oct4 expression, the first differentiation | en_US |
