| dc.description.abstract | Introduction:
Streptococcus suis is a severe zoonotic pathogen that capable of causing severe
invasive disease in human. Here we report the presence of S. suis and S. suis
serotype 2 in Tiet canh samples.
Methods:
30 Tiet canh samples were collected from 3 provinces and then processed and
analyzed. DNA was extracted by using modified DNeasy Blood and Tissue kit. The
presence of S. suis and S. suis serotype 2 was detected by DNA amplification using
16S rRNA gene conventional PCR and cps2J gene specific Real time PCR,
respectively. The isolation of viable S. suis strain was performed by Bacterial
culture.
Results:
In total, the presence of Streptococcus suis DNA was identified in 28 of 30
(93.33%) samples by DNA amplification method targeting at the 16S rRNA gene.
The presence of S. suis serotype 2 was found in 14 of 30 (46.67%) tested samples.
Viable S. suis strains were cultured from 6 (20%) Tiet canh samples.2
Conclusion: We reported the presence of viable S. suis and S. suis serotype 2 in
Tiet canh, which for the first time provides evident to demonstrate that Tiet canh is
as a possible source of human S. suis infection.
Key words: Streptococcus suis, Tiet canh consumption, fresh blood pudding, risk,
PCR, Real time PCR, Bacterial culture isolation | en_US |
