Isolation of sulfur oxidizing bacteria (SOBs) for environmental remediation applications

Abstract

This study focus on isolation sulfur oxidizing bacteria (SOBs) from alum soil and activated sludge. 11 strains of bacteria were isolated and tested sulfur-oxidizing abilities. The experiment was designed for 11 strains of bacteria for evaluating the thiosulfate and Dimethyl sulfide (DMS) oxidizing ability. The strains S3, W4, W7 were selected due to its best Dimethyl sulfide (DMS) removal over 3 days incubation. In additionally, the concentration of sulfate (SO42-) produced also supposed that all bacteria has influenced in thiosulfate oxidizing into sulfate (SO42-). However, its change had not shown drastically. The selected bacteria strains for identification were based on its results of DMS removal ability (S3, W4, W7). Based on BLAST analysis of the partial 16S rRNA gene sequence indicated the identity of strain W7, S3 and W4 as a strain of Microbacterium hominis, Bordetella trematum and Ochrobactrum intermedium, respectively. Keyword: Sulfur oxidizing bacteria (SOBs) Dimethyl sulfide (DMS) Thiosulfate oxidizing Microbacterium hominis Bordetella trematum Ochrobactrum intermedium