| dc.description.abstract | Breast cancer is the most common cancer in women over the world and is the
fifth cause of death from cancer overall. Rs3817198 is a single nucleotide
polymorphism (SNP) that occur on LSP1 gene, which plays a certain role in breast
cancer development and is a T> C SNP. There were many studies shown the
significantly correlated between this SNP and breast cancer in White and European
population, but not been studied yet in Vietnamese. In this research, a HRM method
was developed to detect this SNP in 107 Vietnamese breast cancer patients. The PCR
reactions were optimized at various conditions, the best parameters for HRM assay
to genotype SNP rs3817198T>C was successfully determined, including Ta=64oC,
0.3µM each primer, 20ng DNA and 2.5mM MgCl2. Regarding genotype frequency,
wild-type (TT), homozygous mutant (CC), and heterozygous mutant (TC) were 69%,
2%, and 29%, respectively. Likewise, the risk allele C was calculated to be 16.5%.
The frequency of this SNP in 107 cases is primary estimation that will support for
further association study to find out the suitable markers for diagnosis of breast
cancer in Vietnamese.
Key words: LSP1 gene, rs3817198T>C polymorphism, High Resolution Melting
(HRM) | en_US |
