| dc.description.abstract | Chili pepper is a member of capsicum species, in which male sterile chili pepper is
rarely found in the nature; however, it has been commercially used for F1 hybrid
seed production. Chili pepper has been known to be recalcitrant to differentiation
and regeneration under in vitro conditions. In this thesis, seeds of male sterile chili
pepper were used as a source to produce in vitro plants. The micropropagation of
these plants has been studied using both photomixotrophic (conventional) and
photoautotrophic methods and designed in four experiments. Firstly, chili fruits were
surface sterilized by javel (25-100%, v/v) or burned on the flame of alcohol lamp,
then cultured under different lighting conditions (light or dark). No contamination
rate and the highest survival rate (66.7%) were found in the treatment where fruits
were burned on the flame of alcohol and cultured under lighting condition was the
best. Secondly, nodal cuttings, with no leaf, were cultured onto the agar-based MS
medium supplemented with sucrose (30 g L-1), Morel vitamins, 2 mg L-1 AgNO3, BA
(3-4 mg L-1), IAA (0-0.2 mg L-1). Most shoots were induced in the MS medium
supplemented with BA 4 mg L-1 and IAA 0.2 mg L-1. Thirdly, different culture
methods (photoautotrophic or photomixotrophic) and different supporting materials
(agar or vermiculite) affecting the growth of in vitro chili plants were studied. Chili
plants were proved to grow well under photoautotrophic condition on agar as
supporting material. Fourthly, macro elements in MS medium was altered to
promote the photosynthetic ability and growth of in vitro chili plants; 2 levels of
total nitrogen contents (40 or 60 mmol L-1) was combined with 3 different ratios of
NH4+/NO3- (1:2, 1:3, 0:1). Among chili plants grew the best on the MS medium that
contains 40 mmol L-1 total nitrogen contents with a ration 1:3 of NH4+/NO3. The
results showed that male sterile chili pepper could be successfully multiplied under
photoautotrophic condition.
Keywords
Chili pepper, javel, photoautotrophic methods of male sterile chili pepper, net
photosynthetic rate, nitrogen content. | en_US |
