Study on biochemical activities of components from codonopsis Javanica cultivated in Lam Dong Province

Abstract

Codonopsis javanica (Blume) Hook.f. (known in Vietnam as “Đảng Sâm”) is one of the most precious medicinal plants of Vietnam and have been in the Red Book of Vietnam since 2007. Dried and torrefied Codonopsis javanica (Blume.) roots are used to as medicines. However, a lack of research on the properties of this material medicine has been consistent for years up to this day. Codonopsis javanica (Blume.) root powder is extracted by the reflux extraction method using Ethanol 98% for total extract. This total extract was then extracted by liquid-liquid partition to obtain 4 fractions: diethyl ether, ethyl acetate, n-BuOH and water. Afterwards, the extracts are tested on their antioxidant properties using the scavenging activity on DPPH free radicals method. Next, column chromatography is used to purify individual chemical compounds from mixtures of compounds, checked by TLC, and indentify the structure of the purified chemical compounds by UV spectrum and NMR. The antioxidant responds of fraction was EtOAc (IC50 = 280.46 μg/ml) is highest. From EtOAc fraction, a highly - purified chemical compound is extracted using column chromatography technique, and is structurally indentified as Lobetyolin From n-buOH fraction, column and preparative thin – layer chromatography are utilized to isolate a chemical compound with a high level of purification, and structurally identify the compound as 5 – hydroxymethylfurfural. Key words: Codonopsis javanica (Blume.), antioxidant, column chromatography, UV, NMR.