| dc.description.abstract | Objective: Cancer has been considered as one of the world leading causes of death. Recently, Paramignya trimera plant is a popular Vietnamese medicinal herb used as alternative medicine for cancer treatment support with minimal side effect. However, there are little scientific evidences exists so far. This study aimed to demonstrate the toxicity of methanolic extract of Paramignya trimera on Vietnamese breast cancer stem cell line VNBRCA1 in vitro. Methods: The cytotoxicity of extract on VNBRCA1 and control cell line human fibroblast was determined by the MTT assay and Hoechst 33342/ PI staining technique. Mode of cell death caused by plant extract on VNBRCA1 was studied by flow cytometry (FCM) and the morphology of nuclei of treated-cells was observed by fluorescent microscopy. Results: MTT assays showed that that Paramignya trimera methanolic extract is selectively cytotoxic on VNBRCA1 cell line compared to human fibroblast. Furthermore, FCM analysis of Annexin V/7AAD staining showed apoptosis was dose-dependently induced in plant extract-treated cells, while necrosis was barely induced. Disintegrated nuclei of treated-cells, a phenomenon of apoptosis, were detected by fluorescent microscopy followed by Hoechst/PI staining. Conclusions: These results showed Paramignya trimera methanolic extract strongly killed VNBRCA1 cell lines, indicated that Paramignya trimera methanolic extract may implicate potential agent for cancer treatment.
Keywords: Medicinal plant extract, Anticancer, Paramignya trimera, Vietnamese breast cancer stem cell (VNBRCA1), apoptosis | en_US |
