| dc.description.abstract | The partition coefficients (Kp) of fluoxetine, a well prescribed SSRI
antidepressant between saturated neutral charge 1,2-dipalmitoyl-sn-glycero-3-
phosphocholine (DPPC), saturated negative charge 1,2-dipalmitoyl-sn-glycero-3-
phosphoglycerol (DPPG) large unilamellar vesicles (LUV 100 nm) and water were
determined over the temperature range of 25 -37 by using the second
derivative spectrophotometric method. The van’t Hoff analysis of the
temperature dependence of Kp value revealed the positive =H and positive =S
suggesting an entropy driven mechanism for fluoxetine partitioning into DPPC
and DPPG lipid bilayers. Fluoxetine was found to have an increase in the partition
coefficient with increasing temperature in two kind of lipids used in this study.
The partition coefficient of fluoxetine into DPPG is higher than into DPPC
suggesting the positively charged drug molecules are highly accessible to the
negative charge DPPG. An in-depth study of the thermodynamic characterization
of fluoxetine can shed light onto understanding the energetic forces driving
binding interactions and it essential for understanding and optimizing molecular
interactions of fluoxetine into lipid membranes, that is important key for drug
design.
Keywords:
Fluoxetine, DPPG, DPPC
Thermodynamics
Partitioning
Van’t Hoff method
Second derivative spectrophotometer
1 | en_US |
