| dc.description.abstract | Quality of mature oocytes has an important role in in vitro fertilization (IVF) and
enhances the blastocyst formation. Thus, good mature oocytes induce the normal
fertilization and also promote the developmental competence of the embryo. In
order to achieve high mature oocytes quality with full cytoplasmic and nuclear
maturation, dibutyryl cAMP (dbcAMP) and Follicle Stimulating Hormone (FSH)
were supplemented into the in vitro maturation medium. This study investigated
the effects of dbcAMP 1mM and FSH (0.01IU/ml and 0.02IU/ml, respectively) on
the meiotic competence and in vitro maturation (IVM) sub-sequence in vitro
fertilization (IVF) of fully grown oocytes (4-6mm). Firstly, the suitable
concentration of FSH (0.01IU/ml and 0.02IU/ml) combined with dbcAMP 1mM
were evaluated on the maturation rate and cumulus expansion of the fully grown
oocytes. Although there was significant difference on cumulus expansion and
maturation rate between basic group and supplemented groups (p<0.05) but
these rates were similarly between two concentrations of FSH 0.01IU/ml (74.2%
and 76.3%, respectively) and FSH 0.02IU/ml (65.9% and 74.4%, respectively) in
combination with dbcAMP 1mM. Thus, the suitable combination, dbcAMP 1mM and
FSH 0.01IU, were further studied on the IVM subsequence IVF. The combination
of dbcAMP 1mM and FSH 0.01IU increased the normal fertilization rate
significantly (39.3%) when compared with basic group (24.2%), (p<0.05).
Therefore, this research is strongly claimed that fully growth oocytes cultured in
maturation medium supplemented with combination of dbcAMP 1mM and FSH
0.01IU/mL possessed high maturation rate and normal fertilization.
Keywords— porcine oocyte, in vitro maturation, in vitro fertilization, cAMP,
FSH. | en_US |
