| dc.description.abstract | Adrostenedione has commonly known about the role in regulate the bovine or mice oocyte enclose with granulose cell complexes and promote the meiotic competence at high percentage. However, exactly how androstenedione do so in pig oocyte is unclear. This research is carried out in order to establish the optimal growth culture system for in vitro growth (IVG) of pig growing oocyte and examine the acquisition of meiotic competence during in vitro maturation (IVM) of oocyte after invitro growth with androstenedione. In this study, growing pig oocytes were collected from the early antral follicles (1-1.5mm in diameter) and then were culture for 4 days in a medium containing 10-5mol/L estradiol-17β alone or in combination with 10 ng/ml androstenedione in 96 well plate or floating drop method. In floating drop method, the result showed that androstenedione promoted the oocyte reach fully growth at significant high level (70.2% Germinal vesicle stage) than culture in 96 well plate (50% Germinal vesicle). Then IVG oocytes continue subsequent culture for IVM for 44 hours in floating drop, the outcome indicated that oocyte group supplemented with androstenedione underwent germinal vesical breakdown (GVBD) at higher rate than untreated group (26.1%, 14%, respectively). In conclusion, it is suggested that androstenedione plays a vital impact in promoting the attachment between oocytes and the surrounding cumulus cell as well as enhance oocyte reach high fully growth after 4 days. In addition, floating drop technique has a great effect on preventing oocyte become denuded.
Key words: androstenedione, early antral follicle, growing oocyte, in vitro growth, in vitro maturation. | en_US |
