The effect of the ethanol leaf extract of calophyllum inophyllum L. on cutaneous wound healing

Abstract

Wound healing is a complex biological process in body, is achieved through three phases: inflammation, proliferation, and remodeling. For healing a wound successfully, all three phases must occur in the proper sequence and time frame. During wound healing process, many factors can interfere with phases of this process, thus causing impaired wound healing. So, the necessary of treatment has good effect on cutaneous wound: anti-inflammatory, safety, preventing infection, promoting wound healing in short time, leaving scar with a low cost. In this study, we focus on the effect of oil-extracted Calophyllum inophyllum L.(CIO) in the wound healing. We also compare the effect of CIO with Betadine® and vehicle control. Recent skin models for wound healing are examined in mice to examine the effect of topical treatment. Topical treatment of CIO enhance the wound healing in the examined skin. Male mice, 8-10 weeksold, weight mean 25.5±0.6g, healthy (n=6 in each group) were enrolled. The topical treatments were assessed through day 1, day 7 and day 14. After treatment, the scars or keloids were observed in each group by Hematoxylin and Eosin staining. The wound areas were measured 2.5cm at day 1 and completely recovery at day 7±2 with CIO but extent day 14±2 with control and at day 10±3 with Betadine®. After day 1, 7 and 14 of treatment of different formulations, the mice were sacrificed and the spleen weight, length were taken to determine the different among the different groups. Spleen indexes in the control group, Betadine group and CIO group compare with normal group. Spleen indexes is significant increase in comparing Betadine® group to normal group (P<0.0001 in spleen length, P<0.05 in spleen weight) and vehicle control to normal (P<0.0001 in both spleen length and weight) while spleen indexes of CIO group (P=0.2132 in spleen length, P=0.7703 in spleen weight) has no significant. Base on this study, topical treatment (CIO) has good effect on the rating wound healing or the rate of wound area. Key words: Cutaneous wound Wound healing Oil-extracted Calophyllum inophyllum L. Anti-inflammation