| dc.description.abstract | Neuroblastoma (NB) is pediatric cancer that arises from precursor cells of mature dorsal ganglion neuron which often lead to abnormal proliferative state in young children and infants (younger than 2 years of age). NB is heterogeneous phenotypes therefore it is hard to manage effectively in diagnostic clinics. It is mainly dependent on laborious and time-consuming histology's evaluation. Aside from MYC-N copy status instability of chromosome 1poftenlead to aggressive disease in NB patients. Both of them can be identified by G-band chromosome technique. In this study, I developed a molecular based PCR-technique to identify the loss of somatic 1p36 by using six specific micro-satellite markers for NB. The 8 tumors and its matched-germlines (blood) is assigned to run on a precise 8-10% polyacrylamide gel based on the appearance of specific, size amplicons (PCR products) corresponding to the target micro-satellites. The results showed the optimization of different genomic DNA isolation, PCR set-up, and polyacrylamide gel electrophoresis for the precise identification of small base pair fractions of micro-satellites. Moreover, the elimination of PCR carry-over contamination and erroneous reporting of false-positives were confirmed by CGH-array. Overall, the micro-satellites described in this study are specific for the routine detection of loss of 1p36 in NB samples.
Key words: neuroblastoma, 1p36 chromosome loss, micro-satellite markers, PCR. | en_US |
