| dc.description.abstract | In this study, ultrasound-assisted extraction was used to extract bixin from the annatto seeds. Response surface methodology, using Box-Behnken design was employed to optimize and investigate the effect of three independent variables: seed/solvent ratio (1/15 – 1/25 g/mL), sonication time (30 – 50 min) and extraction temperature (50 – 70oC) on the bixin yield. The experimental data were fitted to a second order polynomial equation using multiple regression analysis with high coefficient of determination value (R2 = 0.9781). The model F-value of 34.76 with low p-value (p < 0.0001) indicated that the model was highly significant and could be employed to optimize the extraction process. Optimal extraction conditions for the bixin yield were determined as follows: seed/solvent ratio of 1/15 (g/mL), sonication time of 50 min and extraction temperature of 54.6oC. Under these conditions, the highest bixin yield was predicted to be 0.912%, which was in good agreement with the experimental value of 0.899 0.021%.
Keywords:
Annatto, bixin, Box-Behnken design, optimization, response surface methodology, ultrasound-assisted extraction | en_US |
