Generation of arabidopsis transgenic plants over-expressing 35S:GmRR34 using agrobacterium-mediated transformation by floral dip method

Abstract

Among the abiotic stresses, drought stress is one of the most adverse environmental factors that plants commonly have to cope with. Drought impact can result in significant reduction in crop productivity worldwide. Recently studies revealed that GmRR34 has an important role in plant responses to abiotic stresses, particularly to drought. Therefore, development of transgenic plants carrying this gene could be considered as a promising approach for improvement of plant tolerance capacity to abiotic stress. In this project, Agrobacterium strain EHA101 carrying recombinant binary vector pBI121-GmRR34 was transferred into Arabidopsis plants by floral dip method. The transgenic seeds from these plants were screened on the germination medium containing kanamycin antibiotic for selection. Transformation efficiency was 0.91% with 8 transformants out of 880 screened seeds. Two of these eight transformants were performed with PCR to confirm the presence of GmRR34. Keywords: Agrobacterium, Arabidopsis, drought stress, floral dip transformation, GmRR34, PCR, transgenic plants