Effects of Lactobacilius Acidophilus - Derived minicells on Taphylococcus Aureus biofilm

Abstract

Development of biofilm is a key mechanism involved in Staphylococcus aureus (S. aureus) virulence during device-associated infections. Moreover, clinical information about genotypically different biofilm-producing (S. aureus is largely unknown. In this study, we aimed to investigate antibiofilm formation ability of Lactobacillus minicells. A biofilm forming reference strain, S. aureus ATCC 25923 was employed. By polymerase chain reaction (PCR), experiments showed that S. aureus TV5 and S. aureus ATCC 25923 were positive for 4 genes (icaAB, finbAB), indicating biofilm production in S. aureus. The results revealed that Lactobacillus minicells could inhibit the formation of S. aureus biofilm on polystyrene surface of 96 well plates. The biofilm formation and anti-biofim were observed by scanning electron microscopy and crystal violet staining. In addition, Lactobacillus minicells (107 cells) could decrease to 31-35% of the biofilm development while Tetracyclin and Trimetoprim showed 37% and 7-19%, respectively in both strains. Lactobacillus minicells could deliver Trimethoprim in anti-biofilm of S. aureus. Therefore, the study clearly demonstrated Lactobacillus minicells could prevent the staphylococcal biofilm development. Keyword: Staphylococcus aureus, biofilm, Lactobacillus acidophilus-derived minicells