| dc.description.abstract | This study was carried to synthesize Heparin – Poly (N-isopropylacrylamide)-terminated (Hep–PNIPAM) copolymer (nanogels/nanoparticle form) as a platform for carrying anti-cancer drugs. The conjugation of PNIPAM and Heparin helped to carry dual drugs, which was used in many regimens for cancer treatment nowadays. Heparin carried for Erlotinib drugs through the electrostatic interaction, while PNIPAM packaged an amount of 5-Flourouracil via hydrophobic interaction when heated to over 32oC. The size and morphology of Hep-PNIPAM copolymer, Hep-PNIPAM-Erlo-5FU nanogels were characterized by Proton nuclear magnetic resonance spectroscopy (1H-NHM), Fourier transform infrared spectra (FT-IR), Transmission electron microscopy (TEM) and Dynamic Light Scattering (DLS) measured, UV-Vis was used to determine the drug loading and releasing efficiency. Hep-PNIPAM with Erlotinib and 5-FU complexes was synthesized at 190 nm and high drug loading efficiency (30.6% and 19.33% of Erlotinib and 5-FU, respectively). After 72 hours of drug releasing at pH 5.5 and 7.4, the results of 5-FU showed there no big differences at different pH (~ 16%-18%). In pH 7.4, Erlotinib releasing efficiency was 39.02% and the higher efficiency in pH 5.5 (53.91%). This would be significant to apply the nanocomplex in cancer therapy due to a low pH at tumor sites.
Keywords: Heparin-g-PNIPAM nanoparticle, Erlotinib, 5-Flourouralcil, combination chemotherapy. | en_US |
