| dc.description.abstract | Embryo transfer is currently standard procedure to producing offspring received from in vitro fertilization (IVF) embryos, transgenic mouse models for research and cloned mouse. Besides, the technique allows the study of the differentiation of embryos during preimplantation development, improve the rate of offspring. In the present study, the effects of in vivo fertilized 2-cells stage embryos transfer compared with in vitro culture blastocyst embryos transfer on the developmental rate to full-term in mice were investigated, and further analyzed the factors affecting to the implantation rate after embryo transfer. In the first experiment, in vivo fertilized embryos at 2-cells stage were collected from the ICR pregnant female then transfer into the oviducts of pseudopregnant female (Oviduct transfer). In the second experiment, blastocyst stage embryos were obtained from in vitro culture of in vivo fertilized 2-cells stages embryos and then transferred into the uterus of pseudopregnant female (Uterus transfer). The results of this study shown that the developmental rate to full-term development in oviduct transfer technique (2-cell embryo) is higher than that in uterus transfer technique(blastocyst) (20.45% and 16.67% respectively). This study confirms the effects of embryo transfer at different development stages on full-term development in mice which may useful for Assisted Reproductive Technology (ART). Furthermore, the embryo transfer system according to a current status of Vietnam has successful established and also full-term development was conducted.
Keywords: embryo transfer, oviduct, uterus, embryo culture, full term development. | en_US |
